FastQCFastQC Report
Sun 21 May 2023
EGAF00007943933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527981
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT40260.7625274394343736No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC39210.7426403601644757No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC32650.6183934649163512No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT25910.4907373560791013No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG23840.4515313998041596No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT18460.34963379364030145No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC18430.34906559137544724No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC18400.348497389110593No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA14520.2750098961894462No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG13640.2583426297537222No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA13370.25322880937003417No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG12470.2361827414244073No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA12320.23334173010013617No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA11850.22443989461741998No Hit
CAGCTCGTCTTCGTCGCCCGCTTCATCTTCGTTGTGCAGCGGGG11160.21137124252577272No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT10310.19527217835490293No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG9760.18485513683257543No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA9630.18239292701820708No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT9610.1820141255083043No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT9200.1742486945552965No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA8840.16743026737704578No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG8440.1598542371789894No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG8280.15682382509976683No Hit
TTACGCGTTGACAATACCGTAGGTCGTCAGCGAGATGTTTCCAT8140.15417221453044713No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT7650.14489157753782808No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG7060.13371693299569493No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC6510.12329989147336741No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG6480.12273168920851318No Hit
GCCACGGCTCAGCTGAGAGCGGATTTTACGGTACTGGCCAGAAC6180.1170496665599709No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG6140.11629206354016527No Hit
ACCGTCTTTGTTCGGCACCTGGGTGTAGTTGGTGTTACGACGTT6140.11629206354016527No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG5850.1107994416465744No Hit
GATTGCCGGAGATGGAGTTTCCGGACGCGCCGGAGATTCCCAGG5700.10795843032230326No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG5650.10701142654754622No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG5450.10322341144851804No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)