FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007943936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499516
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA78821.5779274337558757No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA44010.8810528591676744No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA41280.8263999551565916No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39100.78275770946276No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT26670.5339168314928852No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC24510.4906749733742262No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT24480.4900743920114671No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA17390.34813699661272113No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA15530.31090095212165375No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG12420.24864068418228844No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG12170.24363583949262888No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG12050.24123351404159227No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG11490.2300226619367548No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC11010.22041336013260837No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT10030.2007943689491428No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG9080.18177595912843633No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG8530.17076530081118524No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC8480.16976433187325332No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8360.1673620064222167No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG8320.16656123127187117No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT7730.15474979780427453No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7410.14834359660151028No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG7180.14373913948702344No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC7140.14293836433667792No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6430.12872460541804467No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6000.12011627255183016No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG5960.11931549740148463No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT5890.11791414088837995No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT5880.11771394710079358No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG5730.1147110402869978No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5700.11411045892423867No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG5400.10810464529664716No Hit
CAGCGCGGTGATCCGTTCGACAAAAACTACG5390.10790445150906076No Hit
GCTTCCGAACCGGAGGACAAATCTCCGCGCG5280.10570231984561056No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC5150.10309980060698758No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5100.10209883166905565No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC5080.10169844409388287No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5080.10169844409388287No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5040.10089766894353733No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)