FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007943950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences594663
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA69551.1695699917432225No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC39980.6723135624715174No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA37960.6383447431570486No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA33990.5715842418310875No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG30050.5053282279206878No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA22560.37937453650218694No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT19110.32135848371262377No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG18940.3184997216911091No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC17020.2862125270951783No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG16670.28032684058029506No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT15210.25577511968963934No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG14530.24434007160358048No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC14040.23610011048274404No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC13450.22617852464336943No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG13110.22046100060034002No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG12580.2115483895920883No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT11400.19170521791333917No Hit
CAGCGTCCGCGCGGCGCTCCGACCCCGACCC11300.19002359319480108No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG11220.18867829341997064No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT10590.17808405769318084No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG10480.17623427050278898No Hit
GATGCGGCCTCCAATTCTGCAAACCGTCAGG10130.17034858398790576No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC9960.16748982196639106No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA9930.16698533455082962No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG9880.1661445221915606No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9710.1632857601700459No Hit
ATGTCTGACGAAGGCCCGGGTACCGGCCCGG9430.15857721095813931No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT9380.1577363985988703No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC9190.15454131163364795No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG8650.14546053815354243No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT8520.14327442601944296No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA8370.14075198894163585No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC8200.13789322692012115No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA8090.13604343972972927No Hit
CAGTCCATTGTAAACCGTACTGACCTGGATC7620.12813980355260038No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC7480.1257855289466471No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC7270.12225411703771716No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG7170.1205724923191791No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG7040.1183863801850796No Hit
CCGCGTGTCCTGAGCAACTCCGGTTGGGGTC7010.1178818927695182No Hit
ATGTCTGACGAAGGCCCGGGCACCGGTCCGG6900.11603210557912633No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG6660.11199620625463498No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC6460.10863295681755884No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT6450.10846479434570505No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6360.1069513320990208No Hit
GACGCCGCGTCCAGCAGCTCTGCAGGTCAGT5990.10072932064042996No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)