FastQCFastQC Report
Sun 21 May 2023
EGAF00007943957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404771
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA54401.343969800208019No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA49101.2130315659965758No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC30070.7428891891958663No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG29990.7409127630190898No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA26230.6480207327105943No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA19820.48965958529637743No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT19730.4874361058475039No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA18610.45976613937263294No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT18020.44518999631890627No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC14760.3646506296152639No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT14120.34883922020105196No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA13800.34093351549394596No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG13110.32388683971924864No Hit
AGACGGCGGCGGGGTAGGTTTGACCGGAACTGGTTTCGGCACTG12360.305357844311969No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT11190.2764526114766127No Hit
AACGCAGGCACCGCGACGACGACGGGACGGTGGTGGGGTCGGTT10870.2685469067695067No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA10750.26558226750434194No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT10550.2606412020624007No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT10520.2599000422461095No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG10170.25125317772271233No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG9980.24655916555286816No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT9950.24581800573657697No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC9310.23000659632236498No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT9080.22432437106413256No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG8880.2193833056221913No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA8530.2107364410987941No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG7990.19739556440555275No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG7860.19418387186829095No Hit
ACCCAGCGTGATAGAACGCACGCGGTCAGAGTAACCACATTCCT7180.17738424936569072No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT6790.1677491717539053No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT6760.1670080119376141No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6340.1566317745095375No Hit
CAGAGCGAAAGTAATGTTTTTGCCATCACGCTTAACCTTCAGGT6260.15465534833276098No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT6170.15243186888388743No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA5930.14650259035355795No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA4990.12327958277643408No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC4950.12229136968804583No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG4800.1185855706065899No Hit
GGTACCGCTGTTCAGGGTGTACTGCTGGAACAGCTGCTTCCACG4770.11784441079029871No Hit
CAGGGTTTTCAGGAAAACCAGCGGCGGACGCTTCTGCAGACCTT4460.11018575935528978No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG4290.10598585372963971No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG4280.10573880045754266No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG4220.10425648082496028No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG4170.10302121446447497No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)