FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007943958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404771
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT124363.0723544917990666No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC40941.011436095965373No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT29610.7315247386794015No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA27390.6766789122738536No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG23040.5692107389116314No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT21810.5388231864436929No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20340.5025063554454247No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG20270.5007769825407453No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT18190.4493899019445563No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC15240.3765091866759229No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG14920.3686034819688169No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG14030.3466157407521784No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG12630.3120282826585897No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG11950.2952286601559895No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG11230.2774408245650009No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC10880.2687939600416038No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA10530.2601470955182066No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC10460.25841772261352713No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA10180.25150023099480945No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG10080.2490296982738388No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT9980.24655916555286816No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT9410.2324771290433356No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC9360.2312418626828503No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC9270.22901838323397672No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG9010.22259499815945313No Hit
TCCTACACCAACGGCAAACTGACTATCACCC7790.19245449896361153No Hit
GATGCGGCCTCCAATTCTGCAAACCGTCAGG7460.18430174098440846No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT7120.17590192973310834No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7030.1736784502842348No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7030.1736784502842348No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6570.16231399976776992No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6550.1618198932235758No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6440.1591023072305081No Hit
GGTGACGTTGCTAAAGCGATCAATGACGCGG6270.15490240160485808No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG6020.1487260698024315No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA5340.13192644729983127No Hit
ATGACCCAGGGTCGCCGCGGTAACGTTTACT5190.12822064821837534No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5050.12476190240901645No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC4930.1217972631438517No Hit
GGCACTTCCGCTTTCGGTGATTCCGCGGACC4690.11586798461352221No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG4670.1153738780693281No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG4600.11364450516464866No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC4430.1094445995389986No Hit
ATGGCACTGATCAGCCAGTCCATTAAAAACC4400.1087034397227074No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC4310.10647996027383383No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)