FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007943959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382350
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA27090.7085131424087877No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA24640.6444357264286649No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC21320.5576042892637636No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT19570.5118347064208186No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT19250.5034654112723944No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT17460.45664966653589645No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC16590.4338956453511181No Hit
TTTGTCGATGGTCTGCTCCAGGTTACCAAAGAAGCCTGCTTTTG15180.3970184386033739No Hit
GTAGTTCGGGTCGATATCTGCGACACCTTCGGCGTCCAGCATTT14500.37923368641297245No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT14480.3787106054661959No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC14220.37191055315810123No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA11230.2937099516150124No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA10760.28141754936576435No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG9400.2458480449849614No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC8460.22126324048646526No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT7960.20818621681705243No Hit
GATACCGTCGGTCGGAACGAACGGCGTGCCTTTTTCGAAATATT7890.20635543350333466No Hit
GTCGTGACGTTTCAGCGGGAATGGGCCACCCAGATCGTCGATGG7770.20321694782267555No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT7710.201647704982346No Hit
CTGACCAACACCGGAGAAGTCGCCAACGCCTTTGTTAGCAGAGG7530.1969399764613574No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA7320.191447626520204No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC7310.19118608604681575No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT7280.19040146462665097No Hit
GTTACAGGTTACAGTCAGCGCTTTGTTAACATCAGAACCGGTCA6960.18203216947822676No Hit
GCCCTCAACATCCAGCATTTCCATGTCGCCCAGGGTAAACTTAG6850.17915522427095593No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT6310.16503203870799005No Hit
CTTTGGGTGTTTCTGTTTCTGACGCGCTTCGCTAGAGGTACGTT6280.16424741728782527No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA6140.1605858506603897No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT6050.15823198639989539No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA6040.1579704459265071No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT5800.15169347456518895No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5630.14724728651758862No Hit
GATAACGAAGCTAGCGGAGGTCGGGGTAATGACCACAGAGGACG5480.14332417941676476No Hit
GTAGTCATAGTCACGCGGCAGCAGTACACGTGCGGTAACACGCT5130.13417026284817574No Hit
TGGGATCTGCAGAGTCTGGTTCGGCATGATCAGATAACCACGCA5110.13364718190139924No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG5050.1320779390610697No Hit
AAAGCCATCCTGTTTAGCTTTATTGATGATGTCCTTGATGGAGT4910.1284163724336341No Hit
GCCGTTACCAGCGTACAGCAGAGACACTTTTTCCGGTTCGATAC4610.1205701582319864No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA4380.11455472734405649No Hit
GTCCACAATTTTGCTCGCAGCAGATGGGTCGGCGATTTTATTCA4160.10880083692951484No Hit
GTCGCCATACAGCAGGGAAACTTTTTCCGGCTCGATGTTGGAGT4140.10827775598273832No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG4100.10723159408918531No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT4020.10513927030207926No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC4000.10461618935530273No Hit
GCCCGCACCTTCTTCTTCTTCCTCTTCCTCCAGGCCGATGCTAG3970.10383156793513795No Hit
AACACCGTTGGTGGTACCAACACCTTTGTCCGGAGATTTGGATG3890.10173924414803191No Hit
GTCGATACGAGCGTTCACTTTTTTCGCAACCTGAGTGTAAATTG3870.1012161632012554No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)