FastQCFastQC Report
Sun 21 May 2023
EGAF00007943960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382350
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT63631.6641820321694785No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG61321.6037661828167908No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA42651.1154701190009153No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC22110.5782659866614358No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA19250.5034654112723944No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT19170.5013730874852884No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA18220.47652674251340393No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG17120.44775729044069573No Hit
GAACCGATCTACGCTACCATCGACGACCTGG15570.40721851706551593No Hit
CCGGATTTTGAACAGAACATTGCGACCACCA14670.3836798744605728No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA14600.381849091146855No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT11790.3083562181247548No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA11420.2986792206093893No Hit
GTGAACGCAAAAATTGATCGCCTGAACCAGA9090.23774029030992544No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG8720.22806329279455995No Hit
CTGCCGATCCAGCAGGTCAGCAAAATGGGCG8720.22806329279455995No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA8250.21577089054531187No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG8160.2134170262848176No Hit
AACACCGATGCACACGCTTCCAGCGATGCCC7820.20452465018961685No Hit
ACTAGCCCGGAAGAACCGATCTACACCCAGG7790.20374002876945205No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7640.19981692166862824No Hit
TTCAACAAAAAACAGAGCTCCGACGTAAAAG6860.1794167647443442No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6420.16790898391526088No Hit
CCGCCGCGCACCCCTACCTGGGAATCCCCGG6380.16686282202170785No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG6370.1666012815483196No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6350.1660782006015431No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC6200.16215509350071924No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5530.14463188178370603No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA5380.14070877468288218No Hit
ACTGCACTGTTCGCTCGCGAATACCAGACCC5110.13364718190139924No Hit
GAACGTGGTAACTTCTCCAAATTCACCCTGG5050.1320779390610697No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC4910.1284163724336341No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA4880.12763175101346932No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC4810.12580096769975155No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT4610.1205701582319864No Hit
ACCCTGGGCGACATGGAAATGCTGGATGCAG4200.10984699882306787No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG4030.10540081077546751No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC4000.10461618935530273No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA4000.10461618935530273No Hit
TCCTACACCAACGGCAAACTGACTATCACCC3970.10383156793513795No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA3920.10252386556819668No Hit
GAACCGATCTATGCACAAGTTAACAAGAAAA3850.10069308225447889No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)