FastQCFastQC Report
Sun 21 May 2023
EGAF00007944049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007944049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499099
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT26870.5383701429976818No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC20050.40172390647947603No Hit
TGCACGTTCCGGAGACGGGTTACGGGTGCGTTTGTTAGACGGCG15130.3031462695777792No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT15040.3013430201222603No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC13880.27810113825112853No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA13160.2636751426069778No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT12380.2480469806591478No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT11500.23041520820518574No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA10720.21478704625735576No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC10670.21378524100428972No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC10580.2119819915487709No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA10490.21017874209325205No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC9310.18653613812089387No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA9000.1803249455518845No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT8990.1801245845012713No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT8720.17471483613471475No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG7840.1570830636807527No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC7570.15167331531419617No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA7350.14726537220070568No Hit
ACCAGTAGCAACTACCGCCAGATCGCGGTTCGGGTACGGTGCAA6960.13945129122679067No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC6930.13885020807495105No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA6920.13864984702433786No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA6430.1288321555442908No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT6230.1248249345320267No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC6140.12302168507650788No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT6100.12222024087405504No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC6080.12181951877282864No Hit
AGAACGGGTCAGATCAATCAGGGACATGTCGCAGTGGCGACAGA5970.11961554721608339No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG5870.11761193670995133No Hit
AGACAGGCTTTTACGAGTGGACGCAACCTGTTTACCGACAGCGT5530.11079966098910238No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT5470.10959749468542312No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG5330.10679243997683827No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT5240.10498919052131941No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA5120.10258485791396095No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)