FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007944057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007944057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485771
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT35590.7326497464854839No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT34730.7149459313133143No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC32640.671921543278623No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC30090.6194276727099806No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC27260.5611697692945853No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA26190.5391429294873511No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA23730.4885017837623078No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA23580.4854139090229758No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA20410.4201568228650949No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA19780.40718774895990084No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG19240.396071399898306No Hit
AACAGCGGTAGACGGAGCGGAAGAGGCCAGAGCGCCAGTACCCG18360.37795586809422543No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT17470.35963447797418946No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC16710.3439892459615745No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT16600.3417248044860644No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT15400.31702180657140916No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA14210.29252466697270935No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT11310.2328257553456258No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT11240.23138474713393758No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC11120.22891444734247207No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT10260.21121063217030248No Hit
ATGGTAACGGATTACCTGGTCTTTACGCCAGTCGTCGTGGTATG9550.19659469173746477No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG8270.17024482729516582No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT7360.15151172054321893No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC7070.14554182938051058No Hit
TTCCTCACCGGCAGCTTTTGCCTGTTCTTCAATGTTAGCAACAA6960.14327738790500053No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA6870.14142466306140136No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG6390.13154346389553925No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA6240.12845558915620733No Hit
CTGCAGATAGTCGGCGTTACGGATGTCTTTCGCCGCATCTGCAG6230.12824973084025187No Hit
TTACGCGTTGACAATACCGTAGGTCGTCAGCGAGATGTTTCCAT6220.12804387252429641No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6040.12433842283709813No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT5750.1183685316743898No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC5690.11713338177865701No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT5690.11713338177865701No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC5590.11507479861910241No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG5520.1136337904074142No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC5440.11198692387977051No Hit
AGACGGCGGCGGGGTAGGTTTGACCGGAACTGGTTTCGGCACTG4900.10087057481817566No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)