FastQCFastQC Report
Sun 21 May 2023
EGAF00007944058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007944058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485771
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA72891.5005012649993517No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT58261.199330548756513No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43290.8911606497711885No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC40350.830638304880283No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA38940.8016122823305633No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA35490.7305911633259293No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG35280.7262681386908647No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA24040.494883391556927No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG23520.48417875912724306No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT23040.474297559961381No Hit
CGTCGCCAGCGTGCAATCGACAAACGCCAGC19990.4115107735949655No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG19430.3999827079014597No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC16300.33554905500740057No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT16240.3343139051116678No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG15160.31208120698847813No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG11800.24291281282744337No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA11470.2361194884009132No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT11280.23220818039775942No Hit
AACCCGGAACACCTGAAGGAAGGCACTCAGA10420.21450436522558983No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC10380.213680931961768No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC10020.20627003258737142No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT9220.18980136731093458No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG7320.15068828727939707No Hit
GGTGTGCCGTCTGCCGGCCTGAAGGTTGGCG7180.14780627085602063No Hit
ACTACCGACCCGGATGCAGCGGCTTCCGCGA7020.14451253780073328No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6720.13833678832206944No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC6600.13586648853060393No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6580.135454771898693No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6410.13195518052745017No Hit
TCCTACACCAACGGCAAACTGACTATCACCC6330.1303083139998065No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6100.1255735727328309No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG5820.11980953988607801No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG5720.11775095672652341No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG5670.11672166514674609No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA5510.11342793209145874No Hit
GGCAAACAGGCGCTGGCTTTCGCACGTGAAC5240.10786975756066129No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5110.10519359945324032No Hit
GCAGCTAACGTTGTACAGGTAGTTCAGAAAA4980.10251744134581933No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA4960.10210572471390841No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG4960.10210572471390841No Hit
TTCCCGGTGCTGGCGAACGAGGGCAAATCTG4940.1016940080819975No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)