FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007944099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007944099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483305
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT31140.6443136321784381No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC28600.5917588272415969No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT28110.5816203018797653No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC19430.40202356689874924No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG17050.3527793008555674No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT15580.32236372477007275No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC14490.2998106785570189No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT13140.27187800664176864No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG12710.2629809333650593No Hit
TTTAGCTTCACGACCACGAGAACGAGAGCGGGCGCGACGGGTTG11760.24332460868395733No Hit
GGCCTGTTCCTGGTTGGTAATGGACTGCGGAGTTTCGACTTCAA11290.2335999006838332No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC10810.22366828400285535No Hit
ACCAGTAGCAACTACCGCCAGATCGCGGTTCGGGTACGGTGCAA9860.20401195932175337No Hit
ATCGCTAATCTGTACTTTTTTAGCTTCGCGGCCACGAGAACGGG8800.1820796391512606No Hit
CAGCTCGTCTTCGTCGCCCGCTTCATCTTCGTTGTGCAGCGGGG8480.17545856136394203No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT8330.17235493115113645No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA8240.1704927530234531No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT7520.15559532800198633No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA6990.14462916791673996No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6560.1357320946400306No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA6380.13200773838466393No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT6330.1309731949803954No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG6270.12973174289527317No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA6170.1276626560867361No Hit
GATGCTGGAAATGTTGGAGCGAGATTTAACCAGGGTAACAGAGC5700.11793794808661197No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT5670.11731722204405086No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG5490.11359286578868416No Hit
GTTGTCCTTCAGCAGACGATACAGTTTAGTCGGGGTGCAACCGG5310.10986850953331748No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5150.10655797063965819No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT5110.10573033591624337No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC4870.10076452757575444No Hit
GCCCGCACCTTCTTCTTCTTCCTCTTCCTCCAGGCCGATGCTAG4860.10055761889490072No Hit
AGATTTCATAGCGAATGGCAGGGTCGCAATGTCTGCAATGGTCG4850.10035071021404704No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)