FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007944296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007944296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences529512
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT86041.6248923537143634No Hit
ATGTCCAACATGGACATCGATGGTATTAACA34930.6596639925063077No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA28730.5425750502349334No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26310.49687259212255813No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT17790.3359697230657662No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG15410.2910226774841741No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG15310.28913414615721644No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC14600.27572557373581713No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC14590.27553672060312134No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG13470.2543851697411956No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG13330.25174122588345493No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG12940.2443759537083201No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG12520.23644412213509797No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG12090.22832343742918004No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG11360.2145371587423892No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG11270.21283748054812734No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC11180.21113780235386548No Hit
CCGGCCGTGGCACCAACCAGCGCTGCCTCCC11030.20830500536342897No Hit
ATCGAAGTGATTGACGTTGAAACCACTGAAG10860.20509450210760097No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC10480.19791808306516187No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC10480.19791808306516187No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG10460.19754037679977032No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA9900.1869646013688075No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC9410.177710797866715No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG9320.17601111967245311No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG9010.1701566725588844No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA8930.16864584749731829No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC8030.15164906555469942No Hit
GCACCGACCTCCGCGGCGAGCCGTAAACCAG8020.15146021242200364No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA7840.14806085603347988No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT7550.14258411518530267No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC7270.13729622746982126No Hit
CGTGGCCAGAGCCGTGGCCGTGGCCGTGGTC7240.13672966807173398No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG7240.13672966807173398No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG7120.13446343047938478No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC7050.13314145855051443No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC6830.1289866896312076No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT6540.12350994878303041No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG6500.12275453625224735No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG6460.1219991237214643No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC6320.11935517986372357No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT6030.11387843901554638No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC5900.11142334829050145No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5820.10991252322893531No Hit
AACGGCCCGCACGACCCGCTGCCGCAGGACC5810.10972367009623954No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA5760.10877940443276074No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG5640.10651316684041154No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC5500.10386922298267083No Hit
GCACCAACCTGCAACATCAAAGTAACCGTTT5310.1002810134614513No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)