FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007944326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007944326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences576544
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT68031.1799619803518897No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC29510.5118429816284621No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT21480.37256479991119495No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT21150.3668410390187045No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG17100.2965948826108675No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT16920.29347283121496365No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA16870.2926055947161014No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG16600.2879225176222457No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG16130.2797704945329411No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG15990.277342232336127No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC14850.25756924016206917No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14590.2530596103679858No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT14100.24456069267913635No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA13770.23883693178664592No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC13460.2334600654937004No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC13110.2273894100016651No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC12820.22235943830826443No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG12810.22218599100849198No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG12550.21767636121440861No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG12010.208310207026697No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC11740.2036271299328412No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC11610.20137231503579955No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC11340.19668923794194373No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG10790.18714963645445967No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC10660.18489482155741802No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA9750.16911111727812622No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC9520.16512182938336017No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG9380.16269356718654604No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA8560.14847088860520619No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT7790.13511544652272853No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7460.1293916856302381No Hit
GCACCGACCTCCGCGGCGAGCCGTAAACCAG7170.12436171393683743No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7100.12314758283843037No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7010.12158655714047843No Hit
CCGGCCGTGGCACCAACCAGCGCTGCCTCCC6720.11655658544707777No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6690.11603624354776046No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6630.11499555974912583No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6360.11031248265527002No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA6030.10458872176277959No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG6010.10424182716323473No Hit
ATGGAACCGGTCCAGCAGCGTCCGGTTATGT5990.10389493256368984No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)