FastQCFastQC Report
Sun 21 May 2023
EGAF00007944328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007944328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences586252
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT75281.2840894359422228No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC29830.5088255562454371No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT21870.3730477678540969No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT20910.3566725571938347No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA17860.30464714832529355No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG17750.3027708221038052No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG17590.30004162032709486No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT17220.29373034121845215No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG16900.28827193766503145No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16440.2804254825569891No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG15860.27053212611641414No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC15600.2660971732292598No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA14190.24204608257199978No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13760.23471135279709066No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG13470.22976467457680316No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC13220.22550029680069322No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG13120.22379454569024923No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC12990.2215770692466721No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC12930.22055361858040573No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC11980.20434898303118795No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG11820.20161978125447758No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG11720.1999140301440336No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC11300.19274987548016895No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC10950.18677974659361504No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC10540.17978616704079475No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG10040.17125741148857487No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC9980.1702339608223085No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA9860.16818705948977575No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT8330.14208906749998293No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7940.13543663816925144No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7660.13066053506000833No Hit
CCGGCCGTGGCACCAACCAGCGCTGCCTCCC7350.125372706617632No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7350.125372706617632No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7210.12298465506301044No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6940.11837912706481173No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6910.11786740173167853No Hit
GCACCGACCTCCGCGGCGAGCCGTAAACCAG6830.11650280084332335No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6530.11138554751199142No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)