FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007944331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007944331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences494661
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA18810.38026042077301425No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA17100.34569129161183115No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT15760.3186020325030678No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC14840.3000034366970511No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC13220.2672537353864566No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT12880.26038034128423304No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT12560.2539112644821403No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA10040.20296728466565991No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC10010.20236080871546375No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA9710.19629604921350177No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT9220.18639027536029726No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT9150.18497516480983944No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT8580.17345212175611177No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA8100.16374850655297263No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC7540.15242762214931033No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG7330.14818229049793696No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA7050.1425218482961058No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA6890.13928730989505944No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC6470.13079664659231272No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT6170.12473188709035075No Hit
CAGCTCGTCTTCGTCGCCCGCTTCATCTTCGTTGTGCAGCGGGG5950.120284396788912No Hit
ACGACGGGAGGAGCGAGAGTTCACAGACGGACGACGACCTGGGC5670.11462395458708084No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT5660.11442179593701546No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT5490.11098509888590369No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC5430.1097721469855113No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC5400.10916567103531509No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG5220.10552681533413794No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC5170.10451602208381093No Hit
AACGCAGGCACCGCGACGACGACGGGACGGTGGTGGGGTCGGTT5020.10148364233282996No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)