FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007944340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007944340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497367
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT60391.2141939453160342No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC41980.844044739598727No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41670.8378119175578597No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG26170.5261708155144994No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA24580.4942024702081159No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG23580.4740965926569314No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT21800.43830813061582297No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT19000.3820116734725062No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG17090.34360944734974375No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG16590.3335565085741515No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC15950.3206887469413934No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA14660.2947521649003653No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA14610.29374687102280606No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC12520.2517255869408304No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC11080.22277312326712465No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA10920.21955618285893516No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT10770.21654030122625748No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG10270.2064873624506652No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA10210.20528100979759412No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9910.19924924653223877No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT9850.1980428938791677No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA9820.19743971755263215No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG9460.19020160163420574No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC9240.18577830857294514No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG9180.18457195591987405No Hit
GTGGCAACCAACGAAATGAAGGTTATCAACG9140.18376772081782666No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC9040.18175713306270821No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT9020.18135501551168454No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG9000.18095289796066083No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA8950.1799476040831016No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8840.17773595755247132No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC7430.1493866702053011No Hit
GATATTCGTGTTTACGGCATGAACGGCTGCA7390.14858243510325375No Hit
GTGCCGGTCAGCGGCTCTGCTCCGGGTCGCC6960.13993690775624437No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA6890.13852949632766148No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC6870.13812737877663778No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA6680.1343072620419127No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC6470.13008502775616396No Hit
TCCTACACCAACGGCAAACTGACTATCACCC6470.13008502775616396No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6450.12968291020514028No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6380.12827549877655733No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG6010.12083632408261906No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA5980.12023314775608353No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5870.11802150122545323No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT5780.11621197224584662No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG5720.11500561959277555No Hit
GATGTCCAGTCTGGTGCGATCGTTGAACCGG5520.11098444408253864No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5460.10977809142946758No Hit
GCACCAACCTGCAACATCAAAGTAACCGTTT5400.1085717387763965No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC5350.10756644489883728No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG5330.10716432734781359No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA5260.10575691591923067No Hit
AAATCTGACGCAGTAAAAGCCCTGAAGGAAC5050.10153468163348191No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)