FastQCFastQC Report
Sun 21 May 2023
EGAF00007944356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007944356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450820
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT61091.355086287209973No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50961.1303846324475402No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG25960.5758395812075773No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT23960.5314759771083803No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG23580.5230468923295328No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC18540.4112506099995564No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG15800.3504724723836564No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA14640.3247415820061222No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA11980.2657379885541902No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG11580.25686526773435076No Hit
CAGCGCGGTGATCCGTTCGACAAAAACTACG8650.1918725877290271No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8500.18854531742158734No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG8380.1858835011756355No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC8360.18543986513464353No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA8190.18166895878621178No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT7990.1772325983762921No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7950.17634532629430816No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG7700.17079987578190853No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7610.16880351359744467No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA7410.16436715318752496No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG7390.16392351714653297No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC7360.16325806308504504No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG7310.16214897298256511No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7170.1590435206956213No Hit
TCCTACACCAACGGCAAACTGACTATCACCC6960.15438534226520564No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6810.15105807195776583No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT6760.14994898185528593No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA6480.14373807728139834No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG6080.13486535646155892No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5950.13198172219511115No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA5200.11534537065791226No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG5100.11312719045295241No Hit
TCTATCCTGAAACCAGGTGCTTCTGCTGCCC5080.11268355441196043No Hit
GATGGTGAACGTAACAACCAGGTTCTGGAAG5060.11223991837096847No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC4880.10824719400204072No Hit
GTGGCAACCAACGAAATGAAGGTTATCAACG4830.10713810389956079No Hit
TCTGCTACCGGTCGTTCCGGCACCGTACTGG4800.10647264983807284No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC4780.10602901379708088No Hit
GATATTCGTGTTTACGGCATGAACGGCTGCA4750.10536355973559293No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC4570.10137083536666519No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT4530.10048356328468125No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA4530.10048356328468125No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)