FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007944390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007944390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449989
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT78371.741598127954239No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA62061.379144823540131No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36350.8077975239394741No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA33430.7429070488389716No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA31760.7057950305451911No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC18670.4148990308651989No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC17910.398009729126712No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG16740.37200909355562023No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG15870.3526752876181418No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG15630.3473418239112512No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC14170.31489658636099993No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG13870.3082297567273867No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA13740.3053407972194876No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG13230.294007186842345No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG12680.28178466584738737No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT12520.278229023376127No Hit
ATCCAGGGCAAACTGGAATACCGTCACACCT11620.25822853447528715No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG10990.2442281922446993No Hit
GTTCACGGCCACACCCAGGGTCCGTGGGACC10790.23978363915562378No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC10750.2388947285378087No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT10600.23556131372100206No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG10550.2344501754487332No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA10190.22644997988839727No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC9970.22156097149041423No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC9680.2151163695112547No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG9570.2126718653122632No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC9340.20756062925982635No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG9040.20089379962621307No Hit
GGTCTGCAATCTTCCTCTTACCCAGGTTATG8740.1942269699925998No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC8400.18667122974117145No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC8370.18600454677781011No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG8360.18578231912335635No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA7830.17400425343730624No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC7780.17289311516503736No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA7630.16955970034823073No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA7430.16511514725915521No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT7210.16022613886117215No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG7180.15955945589781081No Hit
GGTCTGCAGAGCTCTTCTTACCCGGGTTATG6980.15511490280873533No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC6720.14933698379293717No Hit
GGCCTGCAGTCTTCTTCTTATCCGGGTTACG6650.14778139021176073No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT6620.1471147072483994No Hit
GTACACGGCCACACCCAGGGCCCGTGGGACC6430.14289238181377767No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC6380.14178124354150878No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG6380.14178124354150878No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA6300.1400034223058786No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC5940.13200322674554268No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5810.12911426723764358No Hit
CCGGCCGTGGCACCAACCAGCGCTGCCTCCC5730.12733644600201338No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC5720.12711421834755962No Hit
CAGAACCTGACCCTGCGTATCCAGGTTTCTA5630.12511416945747564No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC5520.12266966525848409No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA5480.12178075464066898No Hit
GCACCGACCTCCGCGGCGAGCCGTAAACCAG5380.11955847809613122No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA5220.11600283562487083No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC5170.11489169735260196No Hit
TGGCGCCCTGCCCCGTGGACCCCGAACCCGC5170.11489169735260196No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG4680.10400254228436695No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG4650.10333585932100563No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT4580.1017802657398292No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG4500.100002444504199No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)