FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007944392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007944392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472923
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT67701.4315226791676448No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC39630.8379799671405282No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG24660.5214379507869146No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA22900.48422259014681035No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG21850.4620202443103846No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT18750.39647046136474645No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC17440.36877039179739624No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT17250.3647528244555667No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG15950.33726420580094435No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG15200.3214053873463545No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA11890.25141513523343123No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC11240.23767082590612004No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT10090.21335397094241557No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC10000.2114509127278648No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT9370.1981295052260093No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG9290.1964378979241864No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC9210.19474629062236345No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7890.16683477014228532No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG7860.1662004174041017No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA7760.16408590827682307No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7450.1575309299822593No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC7190.1520332062513348No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6380.13490568232037775No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5970.12623619489853527No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC5940.1256018421603517No Hit
CGTGAAACCCTGGCTAAACTGGGCGTGTCTG5750.12158427481852226No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5570.11777815838942068No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG5320.11249188557122405No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA5160.10910867096757823No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG5030.10635980910211598No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA5010.10593690727666025No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG4980.10530255453847666No Hit
TCCTACACCAACGGCAAACTGACTATCACCC4920.10403384906210948No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA4800.10149643810937509No Hit
GCACCAACCTGCAACATCAAAGTAACCGTTT4780.10107353628391937No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)