FastQCFastQC Report
Wed 31 May 2023
EGAF00007944399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007944399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449976
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCGCCGGCGGGCCGGTATCGCTTGGGGATACGGTTGGCGGTG44780.9951641865343929No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG30350.6744804167333368No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC28260.6280334951197397No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT23910.5313616726225399No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT23860.5302505022490088No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG23650.5255835866801786No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG22570.5015823066119082No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA22270.49491528437072196No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA21500.4778032606183441No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA15860.3524632424840436No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT15630.3473518587658008No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA14790.3286841964904795No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG14540.32312834462282436No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT13750.30557185272103404No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT13600.30223834160044094No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT12600.28001493412982026No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG12160.2702366348427472No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG10730.23845716215975962No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG10020.22267854285561894No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT9820.2182338613614948No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG9600.2133447117179583No Hit
GCGGACGGACATCTGAGACGGGGAACGCGGTGGGTTCGGGGTCC9280.20623322132735966No Hit
AACGCAGGCACCGCGACGACGACGGGACGGTGGTGGGGTCGGTT8850.1966771561149928No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT8810.19578821981616795No Hit
CGGGTCCTGGGACCACGGAGCCCACGGATGACGGTAGCACGGGT8770.19489928351734315No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC8640.19201024054616248No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG8610.19134353832204384No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT8520.18934343164968798No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG8410.1868988568279197No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA8390.1864543886785073No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT8270.1837875797820328No Hit
CACAGCCGGCGGACCGGTATCAGACGGAGAGACGGTCGGTGGCG8160.18134300496026456No Hit
TGGGATCTGCAGAGTCTGGTTCGGCATGATCAGATAACCACGCA7410.16467544935729905No Hit
CGGAGCCTGCGGTGGCGGCGGCTCTTGATAACCCTGATACGGCG7170.1593418315643501No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT6720.1493412982025708No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG6470.14378544633491563No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG6420.14267427596138463No Hit
TTTGTCGATGGTCTGCTCCAGGTTACCAAAGAAGCCTGCTTTTG6400.1422298078119722No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA6220.1382295944672605No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA6200.13778512631784806No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG6070.13489608334666736No Hit
GTGCTGGGAACGCGGGGTCCAAGGGCCAGCGTAGCCCGGATAGC6020.13378491297313633No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG5860.13022916777783705No Hit
TGGGGTACCGGATACCTGTTTTTCCAGGCGACCCTGAACCGGAG5720.12711789073195015No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5710.12689565665724395No Hit
CGGTGCCTGTGGCGGAGGCGGTTCCTGATAGCCCTGGTATGGTG5400.12000640034135154No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG5360.11911746404252672No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA5310.11800629366899569No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT5300.11778405959428948No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG5220.11600618699663982No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG4990.11089480327839707No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC4910.1091169306807474No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA4660.10356107881309225No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG4650.10333884473838606No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT4540.10089426991661778No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)