FastQCFastQC Report
Wed 31 May 2023
EGAF00007944400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007944400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449976
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGAAGTGATTGACGTTGAAACCACTGAAG43420.9649403523743488No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC41380.9196046011342827No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39220.8716020409977422No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC32310.7180382953757534No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC25050.5566963571390474No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC24860.5524739097196295No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG24380.5418066741337315No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG23740.5275836933525344No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG23520.5226945437089978No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA23460.5213611392607607No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC18180.4040215478158835No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT18110.4024659092929401No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG17140.380909204046438No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC16720.37157537290877735No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT15700.3489074972887443No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG15570.3460184543175636No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA12790.2842373815492382No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT12770.28379291339982576No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT12450.27668142300922716No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG11520.25601365406154997No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC11250.2500133340444824No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10560.23467918288975412No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG10150.22556758582679964No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10140.22534535175209344No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT9700.21556705246502036No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC9640.21423364801678313No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT9470.2104556687467776No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT9460.21023343467207142No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC9360.20801109392500935No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG9190.20423311465500382No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT8890.19756609241381762No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8820.19601045389087418No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC8580.1906768360979252No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA8190.18200970718438317No Hit
ATTGAAGTTATCGACGTTGAAACCACTGAAG8180.18178747310967697No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC7860.17467598271907836No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA7490.1664533219549487No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC7270.16156417231141218No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6890.1531192774725763No Hit
AGCCCGACCCAACCGATCTCTCACGAAGAAC6720.1493412982025708No Hit
GAACCGATCTACGCTACCATCGACGACCTGG6600.1466744893060963No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6520.14489661670844667No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA6280.1395629989154977No Hit
GGTTACCAGGAACCGCCGGCCCCGCAGGCAC6220.1382295944672605No Hit
TCCCCGACCCAGCCGATCAGCCACGAAGAAC6070.13489608334666736No Hit
CAGACCCCAGAACAGTCTACCCCGTCTCGCA6010.13356267889843015No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5950.1322292744501929No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5820.12934023147901222No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG5740.12756235888136258No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5680.12622895443312532No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA5450.12111757071488258No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG5330.11845076181840809No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5160.11467278254840259No Hit
TCCCACGAGGAACAGCCACGTTATGAAGACC4870.10822799438192258No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG4800.10667235585897915No Hit
CCGTCTGGCTGGTACAGCTATTTTTTCAAAA4760.10578341956015432No Hit
CCGCCTGCGCCGCAGGCGCCGTACCAGGGCT4690.10422778103721086No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC4650.10333884473838606No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC4520.10044980176720536No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)