FastQCFastQC Report
Wed 31 May 2023
EGAF00007944439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007944439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493033
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG23520.4770471753412044No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT17920.3634645145456795No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC12630.2561694653299069No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT12380.2510988108301067No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC12010.24359424217040238No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG10140.20566574651189676No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG9920.20120357055207258No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA8610.17463334097311944No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA8480.17199660063322333No Hit
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT8390.17017116501329527No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC8270.16773725085339114No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT7930.16084116073366286No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG7820.15861007275375077No Hit
TGCGTCTTCGAAATCCTCGTCATCGTCATCTTCTTCTTCACGCA7360.14928006847411837No Hit
ACCTGCGGAGGTAACGTTGTTGTTGGTTTTCGGAGCGGTGGTGT7060.1431952830743581No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG6470.1312285384548296No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT6230.1263607101350214No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC6120.12412962215510931No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5990.12149288181521319No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG5980.12129005563522118No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA5850.11865331529532505No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC5750.11662505349540497No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG5560.11277135607555681No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG5520.11196005135558879No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC5490.11135157281561274No Hit
CAGCTCGTCTTCGTCGCCCGCTTCATCTTCGTTGTGCAGCGGGG5420.10993178955566868No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG5340.10830918011573262No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG5200.1054696135958445No Hit
ACCCGGAGACTGCGGCAGCGTATCACGGGTGCCGTCGCCACCCT5150.10445548269588445No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC5130.10404983033590043No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG4990.10121026381601232No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG4950.10039895909604428No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)