FastQCFastQC Report
Wed 31 May 2023
EGAF00007944458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007944458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407029
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60301.4814669225042933No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA26030.6395121723513558No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC20980.5154423886258719No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG19130.46999108171653614No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT18520.45500443457345796No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG18410.4523019244329028No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG17040.4186433890459893No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG16600.407833348483769No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG14570.35795975225352494No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG12480.3066120595829781No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA12170.29899589464141374No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA12080.2967847499809596No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA11370.2793412754373767No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG11140.273690572416216No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG10800.26533735925450025No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT10460.2569841460927846No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG8900.21865763864491228No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG8620.2117785219234993No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG8570.210550108223247No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8390.20612781890233864No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT8160.200477115881178No Hit
CCGGCCGTGGCACCAACCAGCGCTGCCTCCC7660.18819297887865483No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA7500.18426205503784743No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG7320.1798397657169391No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT7060.17345201447562703No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC7020.17246928351542518No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6900.16952109063481963No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA6670.16387038761365896No Hit
GCACCGACCTCCGCGGCGAGCCGTAAACCAG6670.16387038761365896No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT6620.16264197391340668No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA6370.1564999054121451No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC5740.1410218927889659No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG5740.1410218927889659No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5630.1383193826484108No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG5580.1370909689481585No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC5520.1356168725078557No Hit
TTCACTCCGATCAAGAAACCGGGTACCTCCG5390.1324229968871997No Hit
CCGGCAGTTGCGCCGACCAGCGCGGCTAGCC5200.12775502482624088No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5090.1250525146856858No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA4960.12185863906502978No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC4910.12063022536477745No Hit
GAAACTCTGTATCAGAAAACCGACGCAGGTA4770.11719066700407096No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG4520.11104859850280939No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT4100.10072992342068993No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)