FastQCFastQC Report
Sun 21 May 2023
EGAF00007946259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007946259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485416
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA29450.6066961122006691No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA28150.5799149595398586No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC24900.5129620778878322No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT24660.5080178650889134No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT21470.4423010366366168No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG21070.43406068197175207No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA19160.3947129884470228No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG16820.3465069136575638No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA15770.3248759826622938No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG15700.32343392059594245No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG14810.3050991314666183No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT14110.290678510803105No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA12620.2599831896764837No Hit
GGTATCGGAAGAGTCGTCATCCTCTTCTTCGGAGTCTTCTACCG12180.2509187995451324No Hit
GCGCAGACCCGGGAAACGGTCACGTGCCGCCTGCAGTGCGCGAG12040.24803467541242974No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT11900.2451505512797271No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT11810.2432964714801325No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT11000.22660975328378133No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA10670.21981146068526788No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA9830.20250671588905186No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG9600.19776851195675463No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC8120.16727919969675495No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT7890.1625409957644577No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA7730.15924485389851178No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT7710.15883283616526855No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC7480.15409463223297132No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC7240.14915041943405244No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG7020.14461822436837682No Hit
ACGGTGCGCACGGGTGATAACGTCGTGGTCATCGGAACGGTCGT6550.1349358076371607No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG6490.13369975443743098No Hit
GCGCTGTTTAGCCAGCAGTTCCGCTGCTTTACGTTCTTCCTGCT6460.13308172783756614No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC6460.13308172783756614No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA6260.12896155050513375No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT6220.12813751503864726No Hit
ACGCATTGCGCCGTTAGACGGGTTCAGATAGTACCAAGTGGAAC5980.12319330223972841No Hit
AGTGGTGTGACCCAGGCTTACAACGGAGCCGGTATCGGAAGAAT5920.12195724903999869No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT5740.11824908944080953No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC5170.10650658404337723No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5110.1052705308436475No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)