FastQCFastQC Report
Sun 21 May 2023
EGAF00007946260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007946260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485416
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT68661.4144568782240388No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC33400.6880696145162087No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG25260.5203783970862106No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG22860.4709362690970219No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG20860.42973449577269807No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA19740.40666150271107665No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT17510.36072152545445557No Hit
CGTATGCCGACTACTAGCACCGCTTCCCAGA17210.354541259455807No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG16730.34465283385796924No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG16120.33208629299405046No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT15600.32137383192972624No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT13770.2836742093379699No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG13510.27831797880580783No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC13290.2737857837401322No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12780.26327933154242955No Hit
GGTCCGGGCATTAACCCAATCAGCAAAACCG12370.2548329680109432No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA12300.25339090594459185No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG12270.25277287934472703No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG9800.201888689289187No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9610.19797452082337624No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC9200.18952815729188985No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC8780.18087578489378184No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT8570.17654959869472783No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG8500.17510753662837647No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG8240.1697513060962144No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8040.165631128763782No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7870.16212897803121448No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG7770.16006888936499827No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7530.15512467656607942No Hit
CATTCTAACGATCAGCACGCGGGTGATCACA7240.14915041943405244No Hit
CAGTCCCAACCGCAGCGTCGTAAACGTCGCG6730.13864396723634986No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA6630.13658387857013365No Hit
AGCACCCCGCGTGCGGCTGTCACCCAGACGG6300.1297855859716202No Hit
GCTATGCAGACCGGTTGGTACAAAGTAGGCA6120.12607742637243108No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5830.1201031692404041No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5550.11433492097499877No Hit
AGCAACAAAACCGAAACTCCGTCCGAGACTG5420.11165680570891773No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG5210.10733061950986371No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC5130.10568254857689074No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC4990.10279842444418807No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC4970.10238640671094484No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4960.10218039784432321No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)