FastQCFastQC Report
Sun 21 May 2023
EGAF00007946264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007946264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483710
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA76161.574497116040603No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41770.8635339356225837No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT40840.8443075396415207No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA38370.7932438857993427No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA33770.6981455830973103No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC29880.6177254966818961No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT21490.44427446197101567No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG20770.42938951024374106No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA18780.3882491575530793No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT15800.326641996237415No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC13580.2807467284116516No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA12920.2671021893283166No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC12330.25490479832957763No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12010.2482892642285667No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT11360.23485146058588824No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11100.22947633912881685No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA10640.21996650885861363No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC9950.2057017634533088No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9610.19867275847098467No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9430.19495152053916603No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG9070.18750904467552873No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG8210.16972979677906183No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG8100.1674557069318393No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC7560.15629199313638337No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7020.14512827934092742No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6960.14388786669698786No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC6920.1430609249343615No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG6840.14140704140910876No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6660.1376858034772901No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT6250.12920965041036983No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6160.12734903144446053No Hit
TCTGCTACCGGTCGTTCCGGCACCGTACTGG5910.12218064542804571No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5820.12032002646213641No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA5750.11887287837754026No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC5690.11763246573360071No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG5090.10522833929420522No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG5080.10502160385354861No Hit
AGCCTGGGTTCCCTGAGCTCTAACCCAAGCT5020.10378119120960907No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5010.10357445576895247No Hit
GCACCAACCTGCAACATCAAAGTAACCGTTT4990.10316098488763929No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)