FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007946268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007946268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497100
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT35060.7052906859786763No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG24090.48461074230537116No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT23590.47455240394286863No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA20550.41339770669885334No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA19190.38603902635284654No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG18910.3804063568698451No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG16290.3277006638503319No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC14850.29873264936632465No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG13920.28002414001207004No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG13300.2675518004425669No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG13190.26533896600281637No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA12270.24683162341581172No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG11630.23395695031180852No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT10680.2148461074230537No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG10390.20901227117280227No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10270.20659826996580166No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10250.20619593643130155No Hit
CGTACTATCGAACGTCTGCTGGAAGATCCGC10160.2043854355260511No Hit
ATGTCCAACATGGACATCGATGGTATTAACA9750.19613759806879905No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9600.19312009656004828No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA9480.19070609535304767No Hit
TCTGCCTGCAACGATTTCAGCGTTCGTATGC9080.18265942466304566No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8780.17662442164554415No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC8730.1756185878092939No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG8060.16214041440354052No Hit
GACATGGCAGCTCCGCCAACCCTGCCGCAGC7870.15831824582578957No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG7500.15087507543753773No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG7430.14946690806678736No Hit
GGCAAATCCCTGACCACCTCTATTGTTGGTC6930.13940856970428486No Hit
TCCTCCAACAGCCCGCACGTGGTTAAAACCG6840.13759806879903438No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC6780.1363910681955341No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC6780.1363910681955341No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA6730.13538523435928385No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG6630.13337356668678335No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG6540.13156306578153287No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6410.12894789780728222No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG6120.12311406155703078No Hit
AACGGTCGTAACGCGTCCAAACGTCGTGCCT5900.11868839267752967No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC5790.11647555823777911No Hit
GTTGTTCCGCCGGGTGCCCCGGCAACTTGCG5740.11546972440152888No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT5650.11365922349627841No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA5530.11124522228927781No Hit
ATGCTGTACGCCTTCAAACTGGGTCGCAAAC5210.1048078857372762No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG5180.10420438543552604No Hit
GGTGACCTGGTCGAATACATCATGAACTATA5050.10158921746127539No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG4990.10038221685777508No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)