FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007946280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007946280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491863
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT70141.426006835236641No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT31800.6465214907403077No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC18600.378154079489614No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC15580.3167548687337734No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG14630.297440547469519No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG14270.29012143625359094No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG12900.2622681519040871No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC12890.2620648432592002No Hit
ATGTGCGAAGACACCATCACCTACGAATGTC11560.23502479348924396No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG10650.21652370680453706No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC9630.19578622502607435No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG9620.19558291638118744No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG8970.18236785446353965No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8870.18033476801467074No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT8600.17484543460272475No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC8430.1713891876396476No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG8070.1640700764237196No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG7950.16163037268507693No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7890.1604105208157556No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA7770.15797081707711294No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT7660.15573442198335716No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG7320.14882192805720293No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG7080.14394252057991758No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6850.13926642174751913No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC6750.13723333529865023No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA6670.1356068661395551No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT6180.12564474254009755No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA6020.12239180422190732No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG5850.1189355572588302No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT5830.11852893996905642No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG5760.1171057794548482No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5690.11568261894063997No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5570.11324291520199731No Hit
CGTGAAACCCTGGCTAAACTGGGCGTGTCTG5490.11161644604290219No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5060.10287417431276595No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA4990.10145101379855773No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)