FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007946282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007946282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences531127
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT56911.0714951414633411No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC36230.6821344047657152No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG22770.4287110239170669No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT21780.41007141418154225No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA20450.3850303223146253No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA20430.38465376454219047No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT19490.366955549237753No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG18210.3428558518019231No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA15240.2869370225953491No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG15060.28354800264343555No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG13620.256435843028127No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12800.24099697435829848No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA12590.23704311774773262No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG12000.22593466346090482No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG11820.22254564350899125No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG10960.20635365929429308No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10870.20465914931833631No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA9310.17528764306841865No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG9060.17058067091298315No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC8920.16794476650593926No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT8510.16022533217102503No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC8410.1583425433088508No Hit
CGTAAAGCTAAACCGGCAACTAAGCCGGCAG8280.15589491778802433No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG8200.15438868669828495No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC8200.15438868669828495No Hit
GCCGAGGGTCTGAAGGACGCGATTAAAGACC8010.15081138786015397No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA7990.15043483008771913No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC7960.14986999342906687No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7960.14986999342906687No Hit
GATGTCCAGTCTGGTGCGATCGTTGAACCGG7940.14949343565663203No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG7890.14855204122554494No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG7690.14478646350119653No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7580.1427153957528049No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC7500.14120916466306552No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7490.1410208857768481No Hit
GCACCAACCTGCAACATCAAAGTAACCGTTT7360.13857326025602162No Hit
GAAGATGGCAACAAACCGGGTAAGGAAGACG7210.1357490769627603No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG7050.13273661478328158No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG6780.12765308485541124No Hit
GCTATTAAAGATCTGGTGATGACGAAACCGG6710.12633513265188928No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC6620.12464062267593248No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC6440.12125160272401893No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6100.11485012059262661No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5940.1118376584131479No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5760.10844863846123433No Hit
GCACCGACCTGCAACATCCGTGTGACCGTTT5730.10788380180258206No Hit
ATGTCTGACGAAGGCCCGGGCACCGGTCCGG5620.10581273405419042No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)