FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007946299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007946299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405926
Sequences flagged as poor quality0
Sequence length44
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT52891.3029468425279485No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG34810.8575454639515577No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA26210.6456841887437612No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG25290.6230199593029271No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT24400.6010947808221203No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT24060.592718869941812No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT23380.5759670481811956No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC23290.573749895301114No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC18530.45648714297679877No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA18490.45550174169676244No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG18200.4483575824164996No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA17270.4254470026556565No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA16970.41805649305538445No Hit
TTCCATCGGCTGCTGCGGGCTTACCTGGGTCAGCTGCGGTGGCT16860.4153466395352847No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG16780.41337583697521224No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT14500.35720796401314525No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA14090.3471076008927736No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG13300.32764592561205735No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC12350.3042426452111961No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG11910.29340323113079725No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC11690.2879835240905978No Hit
CCACTGTTCCGGAACCAGTGGACCTTCCATCGGCTGCTGAGGCA11610.28601272153052526No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT11130.27418790617009015No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC10330.2544798805693649No Hit
GCGGACGGACATCTGAGACGGGGAACGCGGTGGGTTCGGGGTCC10150.25004557480920164No Hit
GACCGGCCATTCCGGGGTGCGCGGACGTGGACGACCGTGAATAG10110.24906017352916543No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA10080.24832112256913821No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT10020.2468430206490838No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG8980.22122258736814096No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA8880.21875908416805032No Hit
CATGCTGTCCTGCAGCGGCATCGGTGGTGGCGGGAAGCGGGTAG8820.21728098224799594No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT8410.20718061912762425No Hit
GGTACGCTCGACGTGGCAACGTGCCAGCAGCAGACGCAGACCTT8260.20348536432748826No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT8040.19806565728728884No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA7390.18205288648669954No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC6800.16751821760616467No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC6680.16456201376605586No Hit
GGAGATTTCGCTGTTGTCGGACAGGATTTCCTGAGCTTCTGCGG6570.16185216024595617No Hit
CTGAGGCTGGGTCGCCGGAACCGGTGGAACCGGACCCATGCTGG6510.16037405832590176No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC6370.15692515384577485No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG6360.1566788035257658No Hit
CGCCTGCGGGCACGGACGACCGTGAATGGACAGATCCAGTGCTT6340.15618610288574764No Hit
GGTACCGCTGTTCAGGGTGTACTGCTGGAACAGCTGCTTCCACG5860.1443612875253125No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT5600.1379561792050768No Hit
GCTCGGCTCCTGCTGCATCTGGGTGATTTCGCGGCCAGCACGCA5580.13746347856505864No Hit
CGGAGAACCGCCCTGACCCATAACCTGAATCGGCTGCAGGACCG5510.1357390263249952No Hit
ACCGCTGAACAGATCGCCGCGGGTTTCGGTCAGTGGACGCACGT5380.13253647216487735No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG5240.1290875676847504No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG5220.1285948670447323No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT5090.12539231288461444No Hit
AACCAGCGGTACCAGGGACAGCTGCAGGCGTGGCGGACCGGTTT5060.12465326192458723No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG5030.12391421096456005No Hit
AGATTCAACATCCGTTTCGCTACCGTTGAAGTGCATGTAGGTGG5010.1234215103245419No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG4970.12243610904450566No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG4840.1192335548843878No Hit
CAGCGCGCGCAGGCCCTCAGCGATGTTTTCGAACTTCGGGTTGG4790.11800180328434247No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT4780.11775545296433342No Hit
ATAATCCCAAGATTCGTCCAGGTCCGCCGGGTCGATGGACGGTG4500.11085764400407958No Hit
CTGCCAACCCGCCGCTGCCTGACGTTCCAGGGCAGCCTGCAGTT4450.10962589240403424No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG4290.10568428728388918No Hit
ATCTTCACCACGGGAACCCGGAACGCCCACTTCGCCACGATCGC4220.10395983504382572No Hit
TTTCACGCATTTCTTGCCGTCCACTACGTAAACGCCGGACGGCA4190.10322078408379852No Hit
TTCCTGGCCTTCTTCACCCTCATCACCGTCACCACCTTCATCGC4120.10149633184373508No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG4110.101249981523726No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC4090.10075728088370786No Hit
CAGTGCACGCAGGCCTTCAGCGATGTTTTCGAACTTTGGGTTGG4080.10051093056369881No Hit
GGAGATTTCAGAGTTATCGGACAGAATTTCCTGAGCTTCGGCAG4060.10001822992368066No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)