FastQCFastQC Report
Fri 21 Apr 2023
EGAF00007946300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007946300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405926
Sequences flagged as poor quality0
Sequence length31
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA52161.2849632691672865No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49131.2103191222045397No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA46301.1406019816419741No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC38090.9383483689145312No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC29700.7316604504269252No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG29180.7188502337864537No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG28340.6981568069056923No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC24980.6153830993826461No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT24100.5937042712218483No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG23480.5784305513812862No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA20870.51413311785892No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC20170.49688859545828545No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT19800.48777363361795006No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA18640.45919699649689844No Hit
TGGCGCCCTGCCCCGTGGACCCCGAACCCGC16560.4079561299350128No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG16410.40426087513487685No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT16290.40130467129476804No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC14890.3668156264934988No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA14740.3631203716933628No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA14720.3626276710533447No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA13690.3372535880924109No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12020.2961130846508969No Hit
GTGGCCCCGCTGCGTGCGTCCATGGGTCCGG11940.29414228209082444No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG11600.2857663712105162No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT10590.2608849888896006No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG10350.254972581209383No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC10030.2470893709690929No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG9760.24043791232884812No Hit
GAAGGCCCGTCTACCGGTCCGCGCGGCCAGG8800.21678828160797783No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8300.2044707656075245No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG8180.20151456176741572No Hit
CCGCACCTGCAGTCTGAAACCGGTCCGCCGC8010.19732660632726162No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC7560.18624084192685367No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7470.18402368904677208No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7160.17638682912649103No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC7090.17466237688642758No Hit
CCGTTCGCCTCTGATTACTCCCAGGGTGCGT6870.16924266984622813No Hit
TTCTCCCAGGTTGCTGACGTAGTCCGTGCAC6730.16579376536610121No Hit
ATCGTCACCCCACCGTCCGCGCGTCCGCGTA6450.1588959564058474No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG6440.1586496060858383No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA6180.1522444977656026No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC6050.14904194360548473No Hit
GGCCGCCCTTCCCTGAAACTGCAGGCTGCCC5930.14608573976537595No Hit
GGCACTTCCGCTTTCGGTGATTCCGCGGACC5610.13820252952508585No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC5510.1357390263249952No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5490.13524632568497705No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5420.13352187344491362No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCTG5360.13204377152485922No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC5240.1290875676847504No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG5170.12736311544468698No Hit
GTTCACGGCCACACCCAGGGTCCGTGGGACC5170.12736311544468698No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5000.12317516000453284No Hit
GAACAGGGTCCGGCAGACGATCCGGGTGAAG4960.12218975872449658No Hit
CTGGAAGAGGTCATCGTAGAAAAAACCAAAC4950.12194340840448752No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG4580.11282844656415209No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA4550.1120893956041249No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA4510.11110399432408863No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA4470.11011859304405236No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC4460.1098722427240433No Hit
GTACTGCCGACCCCGATGCAGCTGGCACTGC4450.10962589240403424No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG4360.10740873952395265No Hit
GGTTACCCAGGTCCGCGCGGTGTCAAAGGTG4300.10593063760389823No Hit
CCGTTCGCAAGCGACTACTCTCAGGGTGCAT4270.10519158664387106No Hit
GCACCAACCTGCAACATCAAAGTAACCGTTT4200.10346713440380759No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4130.10174268216374413No Hit
GAACAGGGCCCGGCGGACGATCCGGGTGAGG4110.101249981523726No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT4080.10051093056369881No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG4080.10051093056369881No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)