FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007946310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007946310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370114
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT60571.6365228010829096No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54721.4784633923601918No Hit
ATGTCCAACATGGACATCGATGGTATTAACA15310.41365633291364284No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC13520.36529285571472575No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG11170.30179890520218094No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT10960.2961249777095705No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC9770.2639727219181117No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG9140.24695093944028054No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC8000.2161496187661099No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG7960.21506887067227937No Hit
TCTGCCTGCAACGATTTCAGCGTTCGTATGC7360.1988576492648211No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG7340.19831727521790582No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG7100.19183278665492254No Hit
GGCCGCTCCGACGCGTCCCATGGCCAGTCTG6100.1648140843091588No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5820.15724884765234495No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT5730.1548171644412262No Hit
CGTATGCCGACTACTAGCACCGCTTCCCAGA5700.1540066033708533No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA5340.14427987052637836No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT5330.14400968350292073No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA4650.12563696590780138No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC4590.12401584376705554No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC4380.11834191627444517No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG4320.11672079413369936No Hit
GGTCAGAAAGGTGGCGCGCAGAAGTCCCAGA4250.1148294849694959No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG4010.10834499640651259No Hit
TCTGGCTACGAAATTGACCACATCGACCTGG3990.10780462235959731No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG3940.10645368724230912No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA3880.1048325651015633No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT3820.10321144296081748No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC3780.10213069486698692No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG3770.10186050784352929No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)