FastQCFastQC Report
Sun 21 May 2023
EGAF00007946324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007946324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403644
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG53461.3244344025923833No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG28070.6954147714322522No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT26520.6570145970211374No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT19670.48731060043008195No Hit
ATCCAGGGCAAACTGGAATACCGTCACACCT18530.45906789150835886No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC16790.41596059894362364No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC13740.34039896542497844No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG13460.3334621597249061No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT11590.28713420737085155No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC11370.2816838600350804No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC10840.26855347781708633No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG10290.2549276094776586No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG10290.2549276094776586No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG9990.24749531765615243No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA9690.24006302583464637No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9200.22792361585951978No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG8290.20537899733428466No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA8270.20488351121285092No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC7870.19497378878417615No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7590.18803698308410383No Hit
AACGACCTGTACCGCCGCGTGATCAATCGTA7200.17837500371614592No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA7120.17639305923041096No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6630.16425364925528435No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6620.16400590619456748No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6440.15954653110166384No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC6020.14914132255155532No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC5620.13923160012288058No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5450.13501996809069378No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5380.13328576666567568No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC5350.13254253748352507No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG5320.13179930830137446No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC5320.13179930830137446No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC5100.12634896096560336No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA5070.12560573178345275No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC5000.12387153035843465No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA4970.12312830117628407No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT4950.12263281505485032No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG4740.11743021077979605No Hit
TTCCCGGTGCTGGCGAACGAGGGCAAATCTG4740.11743021077979605No Hit
CGCGATAACAACATTGAACGTGACAACAATA4370.10826371753327188No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT4350.10776823141183817No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT4310.10677725916897068No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT4140.1025656271367839No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)