FastQCFastQC Report
Wed 31 May 2023
EGAF00007946333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007946333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511422
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCGCCGGCGGGCCGGTATCGCTTGGGGATACGGTTGGCGGTG56981.1141483940855106No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT46370.9066876278298547No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT31280.6116279706387289No Hit
ACCAGTAGCAACTACCGCCAGATCGCGGTTCGGGTACGGTGCAA19090.3732729526692242No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT18500.36173649158620474No Hit
CAGCGTCGGAATGGAGCGACGGGTCTTGATACCGTTGCCGCTTT17720.3464848989679756No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA15230.2977971225328594No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG14740.2882159938367923No Hit
GTTCGGCAGGGTCGGCTGGCCGATCTGGTTGGATTTCGGGATGT11490.22466769126083744No Hit
TTCCAGTTCGGACATACGGTCGTACGGAATAAAGTGGAAGTGAT10670.20863396568782727No Hit
GTCAGTGCCGAAGGACTGACGCAGCTTGGTGCGAACCTGGTTTG10040.1963153716500268No Hit
CACAGCCGGCGGACCGGTATCAGACGGAGAGACGGTCGGTGGCG8790.17187371681312108No Hit
CTGTGCGCGGTCGCCGCTACCCGGAGACACCAGACCGCCCGGCA8630.16874518499399713No Hit
CGGAGACAGCTGAGAGTATGGGATGAAATGGTAGTGATCGCCGT8360.1634657875492255No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG7430.14528119635056763No Hit
TTTAACGTTAGAGGTGAATACGCCACCGAAGCGTGGACCCAGAC6900.1349179346997196No Hit
GTAACCCTGCGCGATTTTAATCTGAGAGACACCGTATTTATCGG6880.1345268682223291No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG6670.13042067020972897No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC6590.12885640430016698No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA6320.12357700685539535No Hit
CAGGGTCGGAATCTGATGACGGGTTTTGCATGCAACACCGTTCT6310.1233814736167001No Hit
ATCCTTCATCAGCAGTTCTTCGGAAAACAGGGCATCATACGGCA6230.12181720770713814No Hit
GCCTTGACGAGATGCGTCGGAAATCTGTTTTTCTTCTTTGACCT6070.1186886758880142No Hit
GATGCTGGAAATGTTGGAGCGAGATTTAACCAGGGTAACAGAGC6020.11771100969453797No Hit
GATTTCGTTAACGATATCGCTGTCCTTCAGCTGATAGTTCGGGT5720.11184501253368061No Hit
AGATTTCATAGCGAATGGCAGGGTCGCAATGTCTGCAATGGTCG5680.11106287957889961No Hit
CACATCTGCGCTCGCAGAGATGCCAGCGAAGTAACCGGTTGCAT5640.11028074662411864No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC5610.10969414690803289No Hit
GTTCAGGCCACGGATCTGACCGCGCATTTTTTCGCTAATGGACA5590.10930308043064241No Hit
GAAGCCCCAAGCGCCCAGACCCTGTGCTTTGTAGGCTTTTACCG5240.10245941707630882No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA5170.10109068440544208No Hit
GGATGGCGGAGTCAGGCCTTTTTCCTTGGCGTAAGCCTGTGCCG5150.10069961792805159No Hit
GGAACCGGATTCACCTACGTACAGGGTAACGCCAGAACCCGCTT5150.10069961792805159No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)