FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007946344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007946344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457320
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT51721.130936762004723No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39530.8643838012770052No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC34540.7552698329397358No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG32600.7128487711011983No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA22870.5000874661068836No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT20100.43951718709000265No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG18430.4030000874661069No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT17710.38725618822706204No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC16230.35489372868013647No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG16060.3511764191375842No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC14770.3229685996676288No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG14370.3142219889792705No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG11750.25693168897052393No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG11510.2516837225575089No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG11080.24228111606752384No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG11020.2409691244642701No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10700.2339718359135835No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA10290.22500655995801627No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10240.2239132336219715No Hit
CTGAAGGCGACCACCACCCACCTGTTCGAAG10210.22325723782034462No Hit
CGTTCTGGCTACTCCTCCCCAGGCAGCCCGG10170.2223825767515088No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA10100.2208519198810461No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA9850.21538528820082217No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC9830.21494795766640426No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG9820.2147292923991953No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG9510.20795066911571766No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9490.20751333858129972No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC9240.20204670690107582No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT8930.19526808361759818No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT8730.19089477827341905No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC8630.1887081256013295No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA8530.18652147292923993No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC8150.17821219277529957No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT8140.17799352750809064No Hit
CGTCACGCACATCGCCAGGGTGTAGGCGGCA7870.17208956529344877No Hit
GATGGCGAACACCGTCGTCACCGTGCCCACC7340.1605003061313741No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG6910.15109769964138897No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6730.14716172483162773No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC6630.14497507215953817No Hit
GGTGGCGTTCACGTTAAGCTGCCGAAACTGA6240.13644712673838888No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG6220.13600979620397097No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC6130.13404180879909036No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6080.13294848246304555No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG5960.13032449925653808No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5960.13032449925653808No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG5950.13010583398932912No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA5850.1279191813172396No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5570.12179655383538879No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC5470.11960990116329923No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5430.11873524009446339No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG5170.11304994314703053No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5120.11195661681098575No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG5020.10976996413889618No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT4960.10845797253564245No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT4660.10189801451937375No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)