FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007946367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007946367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517885
Sequences flagged as poor quality0
Sequence length44
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC37880.7314365158288036No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA35270.681039226855383No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT33730.6513028954304527No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA33040.6379794742075944No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT25150.4856290489201271No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT24860.48002935014530257No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT23910.4616855093312222No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA23900.4614924162700213No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT22910.44237620321113763No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA20090.38792395995249906No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC19900.38425519178968304No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT18630.35973237301717564No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT17850.34467111424350966No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG16880.3259410873070276No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG16450.31763808567539126No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC16120.3112660146557633No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT15370.29678403506569995No Hit
ACCAGTAGCAACTACCGCCAGATCGCGGTTCGGGTACGGTGCAA13330.2573930505807274No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT13130.2535311893567105No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC12380.23904920976664706No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT12100.23364260405302337No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA11230.21684350772854977No Hit
GCCTGCGGTCTGGCCGTTCGCGGCTTCATAAGACCAGGACCAGG11190.21607113548374637No Hit
TACCAGCTCCTGGCCCGGTTTGATCATGTTTTTATCCGCCAGTT11030.21298164650453288No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG10370.20023750446527705No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG10290.1986927599756703No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT9940.1919345028336407No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA9910.19135522365003813No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC9870.19058285140523476No Hit
GGTGCCGTTTGCTTTGGCGATGTCGTTTACGGTATCACCCGGTT9370.18092819834519247No Hit
TTTAACAATCGCTTTAACCGCTTCTTCACCTTCCACCGCAACAA9290.1793834538555857No Hit
CAGCACGACTTTAGACGCTTTGTTACCGTATTTAACAACATAGG9010.17397684814196201No Hit
ACGCACGATTGCTTTGACTGCCTCTTCACCTTCAACGGCAACCA9000.17378375508076116No Hit
GTTCGGCAGGGTCGGCTGGCCGATCTGGTTGGATTTCGGGATGT8980.17339756895835948No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA8840.17069426610154764No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG8270.15968796161309945No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA8190.15814321712349266No Hit
AAAGCCATCCTGTTTAGCTTTATTGATGATGTCCTTGATGGAGT8060.15563300732788166No Hit
GAACGCGTTCTGCTGCTCCTTGTTAAATTTGTTATCAGCTTTTG8060.15563300732788166No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC7850.1515780530426639No Hit
TTCATAGAATGCGTTCTGCTGCTCTTTGTTGAACTTGTTATCCG7490.14462670283943346No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA7400.14288886528862588No Hit
CCAGAAGGTACGGATCTGGTTTTTGCGTTTATCGTTCAGGGCAC7090.13690298039139961No Hit
ACCCGGAGACTGCGGCAGCGTATCACGGGTGCCGTCGCCACCCT7030.13574442202419457No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC6930.1338134914121861No Hit
TTTGACAATTGCTTTGACAGCCTCTTCACCTTCCACAGCGACTA6680.12898616488216497No Hit
TTCCTGGTGATACTCAAAGTAATCAGCTTCACCCACCGGATGGA6360.1228071869237379No Hit
TTTCACGTGTTCATACTTAGAAACATCGAAACCGTCCTGTTTGG6100.1177867673325159No Hit
ACGATCGCGGCCCTGGAAGTTAACTTTCCACTGAGTCGGGATTT6070.11720748814891335No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG6000.11585583672050746No Hit
GAATTTGTTTTGCTGAGCGTCGGCCTTCGGTGCTTGGGAATCAT5920.11431109223090069No Hit
TGGAATGGTTTTGCAGATGGATTTGCACAGCTGGTTGTTACCGC5840.11276634774129392No Hit
TGGGATCTGCAGAGTCTGGTTCGGCATGATCAGATAACCACGCA5720.11044923100688377No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG5660.10929067263967869No Hit
TTTCACGATAGCCTTTACCGCTTCTTCGCCTTCAACAGCGACAA5480.10581499753806348No Hit
CTCTGGATTTAAATATGTCTATCAGGTGGACGGGCCTTCACCTG5390.10407715998725585No Hit
TTCCAGTTCGGACATACGGTCGTACGGAATAAAGTGGAAGTGAT5340.10311169468125163No Hit
GCCCGCACCTTCTTCTTCTTCCTCTTCCTCCAGGCCGATGCTAG5330.10291860162005079No Hit
CGGTTCACCATCCGGACCACCTTCCTGGTGATATTCAAAGTAGT5310.10253241549764908No Hit
CGGTTTATTGCCATCTTCCTTACCCGGTTTGTTGCCATCCTCTT5240.10118076406924319No Hit
GTACATGTGCGGACGGTGCGGATGGTTATCGAAACCGTGCGGGT5200.10040839182443978No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)