FastQCFastQC Report
Sun 21 May 2023
EGAF00007946368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007946368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517885
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC57851.1170433590468927No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42030.8115701362271547No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA41560.8024947623507149No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG36600.7067206039950954No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA36370.702279463587476No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA32810.6335383337999749No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA25800.49818009789818196No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG25800.49818009789818196No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT24860.48002935014530257No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG24770.4782915125944949No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC24730.47751914034969156No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT23120.44643115749635537No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT22340.43136989872268944No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA22160.4278942236210742No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA21760.42017050117304033No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA18350.3543257673035519No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG18310.35355339505874855No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC17690.34158162526429614No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG17680.3413885322030953No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA14140.2730335885379959No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC13350.2577792367031291No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT12610.24349035017426648No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG12600.24329725711306566No Hit
GAAGATGGCAACAAACCGGGTAAGGAAGACG12070.23306332486942083No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG11200.21626422854494726No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG11020.212788553443332No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG10960.21162999507612695No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT10950.21143690201492607No Hit
GAGGATAACAACAAACCGGGTAAGGAAGATG10340.1996582252816745No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC9680.1869140832424187No Hit
AGCCTGGGTTCCCTGAGCTCTAACCCAAGCT9340.18034891916158993No Hit
GTTAAAGTTATCGCGACTGAGGGTGGCGTAC9020.17416994120316287No Hit
GCTCAGCACGATGAAGCACAGCAGAACGCGT8990.17359066201956033No Hit
GTTACCATTATCGCGGGTGAAGGTAACGTCA8750.16895642855074003No Hit
AACGGTTTTGTAGCTTGGGATTCCCAGTACA8740.16876333548953917No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT8240.15910868242949688No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT7880.15215733222626646No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA7780.150226401614258No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC7680.14829547100224952No Hit
GAACAGCAGAATGCGTTCTACGAAATTCTGC7670.1481023779410487No Hit
CACGAAGCTCCGAACATGACCCAGACCGGTA7590.14655763345144193No Hit
CAGAACGCATTCTACGAAATCCTGCACCTGC7450.1438543305946301No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG7360.14211649304382246No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG7250.13999246937061316No Hit
ACCGACGTTCTGGAATCCGCGACTGCAGACA6980.13477895671819032No Hit
CTGCACGACGACGCTAACTTTCTGGAACATG6620.1278276065149599No Hit
GTTAAGGTGGTAAGCGGTGAAGGTAACGTTA6490.12531739671934888No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6480.12512430365814806No Hit
ATGGAAACCCTGCTGATCGACAACGATGGCT6290.12145553549533197No Hit
GGTAAAGAAGACAACAAAAAACCGGGCAAAG6160.11894532569972098No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6070.11720748814891335No Hit
GAAGCGCAGAAACTGAACGACTCTCAGGCCC5590.10793902121127277No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA5500.10620118366046515No Hit
ATCGAGAAAACCGATGAACTGGCAACCCTGC5490.10600809059926432No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC5470.10562190447686262No Hit
GTAACCGTCATTGCGCAGCAGGGTGCAGTTC5460.10542881141566178No Hit
GGTTCTCGTGAGCGTGCACGCGGCCGTGGCC5460.10542881141566178No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)