FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007946382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007946382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492290
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA45200.9181579963029922No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC43090.875297080988848No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA30740.6244286904060614No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT28500.5789270551910459No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA21050.4275934916411058No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT19080.3875764285279002No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA18710.38006053342541996No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG17640.3583253773182474No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG17470.3548721282171078No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16400.3331369721099352No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC15660.3181051819049747No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT15430.31343313900343295No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG12210.24802453838184813No Hit
TCCTACACCAACGGCAAACTGACTATCACCC11810.23989924637916674No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC10100.205163623067704No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG9800.199069654065693No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG9180.18647545146153688No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC9170.18627231916146986No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT8840.17956895325925776No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT8570.17408438115744784No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8470.17205305815677752No Hit
GTGGCAACCAACGAAATGAAGGTTATCAACG8300.16859980905563793No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC8080.1641308984541632No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC7920.16088078165309067No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC7550.15336488655061042No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG7540.1531617542505434No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7150.14523959454792906No Hit
GAAGCAGAAAGCCTGCGTGATGTTATTCGTA7050.14320827154725874No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT6960.14138008084665543No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG6740.13691117024518068No Hit
GGTTACTACCAGCTGCTGCAGGCCTCTCTGA5990.12167624774015316No Hit
GGTAACGATAATTCTACTGCAACTCTGTGTC5810.11801986633894655No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5810.11801986633894655No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5670.11517601413800807No Hit
CTGGGCCATCACGCAGTGCCGAACGGTACCC5310.10786325133559488No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA5230.10623819293505861No Hit
CCGAGCACTAAACCGCGTCCGAAAAACCCTC5160.10481626683458936No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5060.10278494383391903No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5060.10278494383391903No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG4970.10095675313331572No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)