FastQCFastQC Report
Wed 31 May 2023
EGAF00007946394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007946394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411277
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA43581.0596264804499158No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG24320.5913289583419447No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC23830.5794148469279828No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG23400.568959606299404No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG23340.567500735514021No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20690.5030672758262679No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA20500.4984475183392215No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT19340.4702426831551485No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT18200.4425241382328698No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC17980.4371749453531319No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA16910.4111584163471334No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA16830.409213255299956No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG16140.3924362412680505No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG15240.37055317948730415No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT14540.35353302032450146No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG13120.31900641173710176No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG12840.3121983480719807No Hit
ATCCAGGGCAAACTGGAATACCGTCACACCT12780.3107394772865976No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG11710.2847229482805992No Hit
GAACTGGTGACCACCGAACGCAAAACTCCGC11330.2754834333065063No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC11020.26794593424869373No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA10510.2555455325729375No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC9850.23949795393372347No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG9740.2368233574938545No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA9650.23463505131577989No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG9140.22223464964002365No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC9060.2202894885928462No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG8760.21299513466593073No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG8750.21275198953503355No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG7840.19062578262339008No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7820.19013949236159572No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA7730.18795118618352108No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7460.1813862676492972No Hit
TTCGTGGCAAAAGGTGTAGCCGCGGACGCCA7110.17287618806789584No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG6960.16922901110443814No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC6420.15609917403599036No Hit
GAAGATTCCGTCTTCACTTCCACCCGTGCGC6370.15488344838150445No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6280.1526951422034298No Hit
GGCGGCGGTGGCGCAAGCGGTGGCGGCGGTG6260.15220885194163544No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG6200.15074998115625235No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6180.15026369089445799No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA6160.14977740063266365No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA5630.13689070869511302No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG5570.13543183790972993No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC5530.1344592573861412No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG5460.13275724146986093No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC5410.13154151581537504No Hit
GCACTGGGCCAGCGTCGTGCCGATGCAGTTA5380.1308120804226835No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC5330.12959635476819759No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5280.12838062911371168No Hit
GGCACTCTGGATCAGACCAACGGTCTGTTCG5200.12643546806653422No Hit
TTTCTGCAGACTATGCTGCGTAAAGAGGTTA5190.12619232293563706No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC5140.12497659728115114No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5130.12473345215025396No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC5060.1230314362339737No Hit
CAGCGCGGTGATCCGTTCGACAAAAACTACG5020.12205885571038497No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG4850.11792538848513288No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC4780.11622337256885261No Hit
TCCTACACCAACGGCAAACTGACTATCACCC4770.11598022743795544No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC4650.11306248586718927No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG4630.11257619560539489No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT4610.11208990534360055No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG4610.11208990534360055No Hit
GCGCTGGGCCAGCGTCGCGCTGACGCAGTTA4600.11184676021270334No Hit
TCTTCCGCTACTGCATGCACCGCAGGTGTTA4580.111360469950909No Hit
GACGATGTGTGGACCAGCGGTTCTGACTCCG4490.10917216377283435No Hit
GCCGAAGTGCTGGCCCAAGTTGTTAAATCTC4450.10819958324924564No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG4430.10771329298745129No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG4320.1050386965475823No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG4260.10357982576219919No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA4200.1021209549768161No Hit
GGCGCACAGGAAGAGCGCGAAGATACTGTAA4120.10017579392963866No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)