FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007946431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007946431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500252
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT23140.4625668662993851No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT14380.28745512261820044No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG12620.2522728544813414No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC12160.24307748894557146No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG11410.22808504513725084No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT10890.2176902840968152No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG10290.20569632905015875No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA10020.2002990492791633No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG9380.18750549722939638No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG9230.18450700846773224No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA9020.1803091242014025No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT8580.17151355716718775No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG7880.15752060961275516No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG7840.15672101260964474No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA7630.15252312834331497No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT7270.14532675531532108No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG7170.143327762807545No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT7090.14172856880132415No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG7080.14152866955054652No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG6890.13773058378577197No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG6710.13413239727177503No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG6560.1311339085101109No Hit
GTGACGACGGCGGCGTTCACCGCCGTCCGGACCTTCGCCTTCAC6550.1309340092593333No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG6410.12813541974844678No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6280.12553672948833788No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG6240.12473713248522744No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG5930.1185402557111216No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA5830.11654126320334551No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG5770.11534186769867986No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT5650.11294307668934857No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG5600.11194358043546053No Hit
ACCCTCACCGGAACGAGCCGGACGAGAACCTTCACGGTGACGGC5550.11094408418157249No Hit
TACGGTATCAGGGGATTCCGGCTGCACCTCGGTTTCGCCTTTGT5420.10834539392146358No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC5320.10634640141368751No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG5260.10514700590902185No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT5150.10294811415046817No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)