FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007946477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007946477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501472
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT35250.7029305723948694No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC34550.6889716674111416No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC34010.6782033692808372No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC26170.5218636334630847No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT24590.4903563907855274No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA24140.4813828090102738No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA20340.4056058962414651No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA20180.4024152893880416No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG19260.38406929998085637No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT18210.3631309425052645No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT16870.33640961010784254No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC16310.32524248612086015No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT15640.3118818199221492No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA15150.30211058643353966No Hit
CAGCTCGTCTTCGTCGCCCGCTTCATCTTCGTTGTGCAGCGGGG13800.27518984110777867No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG10380.20699061961585094No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG9960.1986152766256142No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT9660.19263288877544507No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT9160.1826622423584966No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT8850.17648044157998852No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT8820.1758822027949716No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG8560.17069746665815838No Hit
GGCCTGTTCCTGGTTGGTAATGGACTGCGGAGTTTCGACTTCAA8000.15953034267117605No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA7950.15853327802948122No Hit
CTGAGCTGCAGCGCTCGCACGCTGGGACGCAGAGAAAGATGCGT7890.15733680045944737No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG7720.15394678067768489No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG7600.15155382553761726No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG7460.14876204454087166No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT7420.1479643928275158No Hit
ACCGTCTTTGTTCGGCACCTGGGTGTAGTTGGTGTTACGACGTT6710.1338060749154489No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT6500.12961840342033054No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA6480.1292195775636526No Hit
ACCCGGAGACTGCGGCAGCGTATCACGGGTGCCGTCGCCACCCT6160.12283836385680556No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG6150.12263895092846659No Hit
TTACGCGTTGACAATACCGTAGGTCGTCAGCGAGATGTTTCCAT6060.12084423457341587No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG5980.11924893114670411No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG5770.11506125965158574No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG5720.11406419500989087No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG5650.1126683045115181No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG5550.1106741752281284No Hit
GATTTCCGGGGTTTTGACAGAACGCAGCAGGTCAGCGATGGCCG5430.10828122008806075No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA5400.10768298130304384No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC5360.10688532958968795No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC5220.10409354859294238No Hit
TTCCTCACCGGCAGCTTTTGCCTGTTCTTCAATGTTAGCAACAA5210.10389413566460341No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)