FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007946478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007946478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501472
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA76441.5243124242230872No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49260.9823080849977666No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC45910.9155047540042116No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA38700.7717280326718142No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA34260.6831886924893115No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA26100.5204677429647119No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT24020.4789898538702061No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG22660.4518696956161062No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT22350.44568789483759813No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA18470.36831567864207776No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG17110.3411955203879778No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC16330.3256413119775381No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG16050.32005774998404696No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC14510.28934815901984556No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11270.22473837023801926No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG11160.22254482802629058No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10780.21496713674940973No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT10550.21038063939761345No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG10340.20619296790249506No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC10270.20479707740412226No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG10150.2024041222640546No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC9640.19223406291876713No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA9640.19223406291876713No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG8300.16551273052134516No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT8030.16012858145619296No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG7910.1577356263161253No Hit
TCCTACACCAACGGCAAACTGACTATCACCC7610.15175323846595623No Hit
ATGGCAATTAAACACACCACTATCGACATCG7570.15095558675260035No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7180.14317848254738053No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC7140.14238083083402464No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT6940.13839257226724522No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6830.13619903005551656No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6790.13540137834216068No Hit
CACGAAGCTCCGAACATGACCCAGACCGGTA6700.13360666198710994No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6400.12762427413694086No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6360.12682662242358497No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5840.11645715014995853No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC5820.11605832429328058No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA5800.11565949843660263No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5600.11167123986982323No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5570.11107300108480632No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC5470.10907887180141662No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC5190.10349530980792547No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA5110.10190000638121371No Hit
ACTACCGACCCGGATGCAGCGGCTTCCGCGA5080.1013017675961968No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)