FastQCFastQC Report
Wed 31 May 2023
EGAF00007946479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007946479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503470
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT35460.7043120742050172No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC34440.6840526744393907No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC34120.6776967843168411No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC25230.5011222118497627No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT24800.49258148449758676No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA24750.49158837666593835No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA21210.4212763421852344No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA20480.40677696784316836No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG18370.36486781734760765No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT17920.35592984686277235No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT16860.33487596083182714No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC16550.3287186922756073No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT15730.3124317238365742No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA15530.3084592925099807No Hit
CAGCTCGTCTTCGTCGCCCGCTTCATCTTCGTTGTGCAGCGGGG13960.2772757065962222No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG10840.21530577790136451No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG10190.20239537608993585No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG9820.19504637813573797No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT9430.18730013704888077No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT9170.18213597632430928No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT8700.17280076270681471No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA8620.17121179017617733No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT8500.16882833138022127No Hit
GGCCTGTTCCTGGTTGGTAATGGACTGCGGAGTTTCGACTTCAA8370.16624625101793553No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG7960.15810276679841898No Hit
CTGAGCTGCAGCGCTCGCACGCTGGGACGCAGAGAAAGATGCGT7790.15472620017081457No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG7630.1515482551095398No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT7130.1416171767930562No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG6760.13426817883885833No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT6600.13109023377758358No Hit
TTACGCGTTGACAATACCGTAGGTCGTCAGCGAGATGTTTCCAT6310.12533020835402306No Hit
ACCCGGAGACTGCGGCAGCGTATCACGGGTGCCGTCGCCACCCT6280.12473434365503408No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA6130.12175502016008898No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA6040.11996742606312193No Hit
ACCGTCTTTGTTCGGCACCTGGGTGTAGTTGGTGTTACGACGTT6020.11957018293046258No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG5960.11837845353248456No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG5860.11639223786918784No Hit
GATTTCCGGGGTTTTGACAGAACGCAGCAGGTCAGCGATGGCCG5780.11480326533855045No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG5750.11420740063956143No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG5610.11142669871094604No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG5570.11063221244562735No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC5510.1094404830476493No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG5450.10824875364967128No Hit
TTCCTCACCGGCAGCTTTTGCCTGTTCTTCAATGTTAGCAACAA5150.10229010665978111No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG5110.10149562039446242No Hit
CAGGTCATCTTCGTCCGCATCGCCGTCTTTGTTCGGCACCTGGG5080.10089975569547341No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)