FastQCFastQC Report
Wed 31 May 2023
EGAF00007946520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007946520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472000
Sequences flagged as poor quality0
Sequence length33
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTATT34440.7296610169491525No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACTGG20860.4419491525423729No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCTAC15370.32563559322033897No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTATC15050.3188559322033898No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCATT14210.3010593220338983No Hit
ATGTCCAACATGGACATCGATGGTATTAACACC12510.2650423728813559No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCCAG9440.2No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTCGT8580.18177966101694915No Hit
GAACGTACCACTGACGGTAAACTGGATCCGCGT8010.16970338983050848No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCCGT6250.13241525423728812No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCAGC5730.12139830508474576No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACACT5370.11377118644067796No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAACAG5300.11228813559322035No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGTTC5270.11165254237288134No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4970.10529661016949153No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTGGT4910.10402542372881356No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCGGT4730.10021186440677966No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)