FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007946727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007946727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478165
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT23060.482260307634394No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC22760.47598632271287106No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT20710.4331140924157979No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC18350.38375874436648433No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC17790.37204730584630824No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA13630.28504804826785735No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT13510.2825384542992482No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG12550.2624617025503749No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT12030.2515867953530685No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA11780.24635847458513274No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA11690.24447627910867586No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG11530.241130153817197No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC11090.23192830926563007No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG10620.22209906622191083No Hit
GGCCTGTTCCTGGTTGGTAATGGACTGCGGAGTTTCGACTTCAA10350.2164524797925402No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT10080.21080589336316963No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG9960.20829629939456046No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA9370.19595746238223205No Hit
GGACTGGTCCGGCAGGGTATCCGGGAAAGCCGCACGTGCCGCAC9080.18989261029142662No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG8300.17358024949546705No Hit
AGTCACGTTACGAGAAGAGGTGGTTTTGCTGGAGGCCGGCTGCG8240.17232545251116246No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG8230.17211631968044502No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT7990.1670971317432267No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT7330.1532943649158763No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT6920.14471991885646168No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG6560.1371911369506342No Hit
GATTTCCGGGGTTTTGACAGAACGCAGCAGGTCAGCGATGGCCG6450.13489067581274244No Hit
CTGGGAGGTCAGGAACACCAGGTAGCTGCGCTGAGCTTCACGGA6350.13279934750556815No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG6290.13154455052126357No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC6280.13133541769054616No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG5940.12422490144615353No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT5920.12380663578471866No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT5860.12255183880041408No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG5560.11627785387889118No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA5530.11565045538673889No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG5520.11544132255602146No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG5180.10833080631162884No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA5180.10833080631162884No Hit
TGGACCGGTAACCGGACGGCCACGACGGCCGTTATCGAACAGGG4890.10226595422082335No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)