FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007946741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007946741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450649
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT23800.5281272120874562No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC23660.5250205814281181No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA16220.3599253521032999No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA15670.3477207316559007No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA15380.34128556814727207No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT15010.3330751871190217No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA14090.3126601856433721No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA13940.3093316527940814No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT12150.2696111607925458No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT12010.26650453013320785No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT10550.23410681040011183No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA10480.23255349507044284No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC10240.22722784251157777No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC9740.21613273301394212No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT9060.20104338409715766No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC8200.18195979576122437No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC8010.17774365415212284No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG7480.16598283808462905No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT7090.15732865267647328No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG6960.154443924207088No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG6940.15400011982718256No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT6840.15178109792765546No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG6740.14956207602812832No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC6590.14623354317883763No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA6590.14623354317883763No Hit
TTTACCACGTTTGCTGCGGCCCTTACGAGACGGCAGTTTGCCAG6090.135138433681202No Hit
ACCTGCGGAGGTAACGTTGTTGTTGGTTTTCGGAGCGGTGGTGT5990.13291941178167488No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC5970.13247560740176945No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC5590.12404332418356637No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG5390.11960528038451211No Hit
CTGACCAACACCGGAGAAGTCGCCAACGCCTTTGTTAGCAGAGG5040.11183870373616717No Hit
ATCAGAGGATTTCGCATCGTTTTCTTTTTTATCCGGGGTTGCCA4910.10895397526678191No Hit
TACGGTATCAGGGGATTCCGGCTGCACCTCGGTTTCGCCTTTGT4810.10673495336725478No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA4740.10518163803758579No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC4550.10096549642848425No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)