FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007946742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007946742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450649
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT36450.8088334823776376No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC33000.7322772268439517No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG24690.5478765069932475No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA23280.516588298209915No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG17350.38500029956795645No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA17010.3774556251095642No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT16060.3563749170640565No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT15070.334406600258738No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG14940.33152187178935266No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12880.2858100206590939No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC11430.25363420311595053No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG11310.250971376836518No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA11180.24808664836713273No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG9540.21169468921488785No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG9040.20059957971725223No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA8870.19682724248805608No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT8770.194608220588529No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC8160.1810721870014135No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8100.17974077386169726No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7500.16642664246453448No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7250.16087908771571666No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7180.15932577238604767No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG7120.1579943592463314No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT7040.1562191417267097No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG6980.15488772858699343No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC6860.15222490230756086No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA6460.14334881470945238No Hit
TACCTGGAGACCCAGCAGAAAATCAACCACC6310.1400202818601617No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC6230.13824506434053999No Hit
ACTCGTCTGGAAAACATCAAAACCGACCGTA5500.12204620447399195No Hit
ACTAGCCCGGAAGAACCGATCTACACCCAGG5470.12138049790413383No Hit
GATGTCCAGTCTGGTGCGATCGTTGAACCGG5310.11783006286489042No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5040.11183870373616717No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4980.11050729059645091No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT4670.1036283227079168No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG4510.1000778876686734No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)