FastQCFastQC Report
Sun 21 May 2023
EGAF00007946784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007946784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470158
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG65411.391234436083189No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT33400.7103994827270832No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA31800.6763683697820733No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT23650.5030223882184287No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC17480.37178990892423397No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA13150.27969320951680077No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA13110.2788424316931755No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC12910.2745885425750493No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG12620.26842040335376616No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG11960.2543825692639496No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA11960.2543825692639496No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG10540.22417995652525324No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG10170.21631026165671968No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC9930.21120559471496814No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG9020.19185039922749372No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC8880.18887267684480535No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG8880.18887267684480535No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8580.182491843167616No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG8460.17993950969674025No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC8370.17802525959358342No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT7930.1686667035337057No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC7800.16590167560692362No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC7390.1571812029147648No Hit
GAACTGCTGATGAAGGACCCGAACTACCAGC7170.15250192488482595No Hit
AGCCTGGGTTCCCTGAGCTCTAACCCAAGCT7050.1499495914139502No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA6870.14612109120763658No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6530.13888947970682197No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA6150.13080709038238209No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6000.1276166735437874No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG5950.12655320126425584No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5800.12336278442566116No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC5740.12208661769022328No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC5620.11953428421934754No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5510.1171946452043781No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG5460.11613117292484654No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5440.11570578401303391No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG5120.10889956142403191No Hit
TACTATAACGGTAAAGTTCCGTATGATGCAC5080.10804878360040668No Hit
CTGCACGACGACGCTAACTTTCTGGAACATG4940.1050710612177183No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA4920.10464567230590567No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT4710.1001790887318731No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)