FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007946810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007946810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences531043
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72391.363166447914764No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT57581.084281310553006No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC29700.5592767440678061No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC19890.37454594072419745No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG19370.36475388998631No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG17680.33292972508817553No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG17410.3278453910511955No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG17410.3278453910511955No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA17170.3233259830183243No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT15340.2888654967676817No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT14850.27963837203390307No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG13610.2562880971974021No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12820.2414117124225345No Hit
GAAGTCTCCATTGCCGAACCGACCACTTACA11950.22502885830337657No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG11740.2210743762746143No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC11560.21768482024996094No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC11070.20845769551618232No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG10410.19602932342578663No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG10410.19602932342578663No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC10100.19019175471666136No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG9750.18360095133539092No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG9180.1728673572573219No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG8630.1625103805153255No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC8190.15422479912172837No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7800.14688076106831272No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7710.14518598305598604No Hit
ATGGACGAACTGCAGCCGACCATGCAACTGA7690.14480936571991343No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7690.14480936571991343No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG7430.1399133403509697No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG7340.13821856233864302No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC7200.13558224098613483No Hit
TCCATCTCTAGCAGCGAGGAAATCGTCCCAA6840.12880312893682808No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA6670.12560188158021102No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6570.12371879489984804No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6550.12334217756377543No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG6490.12221232555555765No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6440.12127078221537614No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA6300.11863446086286798No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC6270.1180695348587591No Hit
TCCGAGCGTTATCTGGGTTACCTGGAACAGC6200.116751374182505No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG6000.11298520082177904No Hit
AACGCTACCGTAGCCCTGCAGGGCGAGGTTC5520.1039463847560367No Hit
AAGGAAAACGACGCTAGCTCCGAATCTGGCA5470.10300484141585521No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC5320.10018021139531073No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)