FastQCFastQC Report
Wed 31 May 2023
EGAF00007946850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007946850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences496633
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA40630.8181091469958702No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT27740.5585613521453467No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT19450.3916372854804252No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA19090.38438847197024767No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC17450.3513660993127722No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA17110.3445199976642712No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG16760.33747254008493194No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC16070.32357898085709164No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG16000.3221694893412238No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG14890.29981898101817644No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG14160.28511999806698307No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG13990.28169694724273253No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC13680.2754549133867463No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA13170.2651857609139948No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG12390.2494799983086102No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12320.24807050679274234No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG12200.24565423562268313No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG12140.24444610003765355No Hit
GCCGGTCAGCCGAGCGAACCGTGGCCGGATC11920.24001626955921174No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC10960.2206861001987383No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC9490.19108677836551338No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT9460.19048271057299856No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC9210.18544881230204194No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG8410.16934033783498076No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG8020.16148745653228844No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA7900.15907118536222925No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG7510.1512183040595369No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG7170.14437220241103593No Hit
ATGTCCAACATGGACATCGATGGTATTAACA7090.1427613549643298No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT6790.13672067703918184No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG6770.13631796517750533No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC6690.13470711773079921No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG6670.13430440586912265No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG6380.128465083874813No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG6250.12584745677391554No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5970.12020949071044414No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG5960.12000813477960587No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5780.1163837280245171No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5760.11598101616284055No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG5740.11557830430116403No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG5710.11497423650864926No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG5550.111752541615237No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC5530.11134982975356048No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA5460.10994033823769263No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC5180.10430237217422121No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)